Life Science Optics

Filters for Flow Cytometry

Flow cytometry is a technique for analyzing a large population of fluorescently labeled cells in a fluid stream. As the particles pass through a focused light source the amount of light scattered and the emission of a fluorescence label can be measured. Flow cytometry is a very important tool in basic research for investigating many aspects of cell functions and has emerged as a very important clinical technique in the areas of blood cells and cancer diagnostics.

Flow cytometry

Why consider Semrock filters for your flow cytometer?

With high-performance flow cytometers moving toward multiple lasers and detection channels at both visible and near-IR wavelengths, having the right optical filters is essential to discriminating between more fluorophores more completely. Semrock optical filters can help you achieve better sensitivity with the highest transmission of any filters on the market. Our outstanding selection of laser clean-up filters allows you the option of an ultra-narrow bandpass for gas or DPSS lasers or wider bandpass filters for systems with either multiple lasers at similar wavelengths or diode or optically pumped semiconductor lasers whose output can vary appreciably from laser to laser, with temperature or as they age. Semrock BrightLine® emission filters provide exceptional laser-line blocking (OD>6) to ensure no laser light reaches the photomultiplier tubes dedicated to precious signal photons and are optimized to eliminate crosstalk between popular fluorophores.

Introducing our New Family of Optical Filters for the Flow Cytometry Market

Spectral flow cytometry may require a balance between instrument cost and filter performance. IDEX Health and Science understands these needs, and we are proud to announce our latest Semrock line of optical filters, Nanopede, which cover the NUV and visible spectrum in 20 nm Full-Width, Half-Max (FWHM) steps. The filters in the Nanopede family have been designed with a value approach to optical performance, providing essential filter performance of OD>5 from 330 to 900 nm.

Learn More about the Nanopede Family

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Learn more about flow cytometry:

Technical Notes

Optical Filters for Laser-based Fluorescence Microscopes

Laser Damage Threshold

White Paper

Optical Filters for Laser-based Fluorescence Microscopes

Related Publication

Orange Laser Sources for Life Sciences Research  from the January 2010 issue of Biophotonics 

Additional Resources

Cytometry Webinars - Molecular Probes® Flow Cytometry Webinars