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View All Resources

  • Laser Damage Threshold

    by IDEX Health & Science
    Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Laser damage may be broadly classified into two types: absorption-driven and dielectric-breakdown damage. Which type dominates depends on the material properties (absorption coefficient, specific heat, melting temperature, as well as defects that cause scattering and concentrated electric field effects), geometric properties of the sample (thickness, homogeneity, surface morphology, etc.), and of course the properties of the laser beam itself. Full story

  • How to Calculate Luminosity Dominant Wavelength and Excitation Purity

    by IDEX Health & Science

    In this article we briefly describe the method to calculate the three main parameters that fully specify color in this system: luminosity, dominant wavelength, and excitation purity.
    Full story

  • Leica Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to Leica DM-K, DM-R and DM-IRB cubes.
    Full story

    leica cube assembly guide thumbnail

  • Zeiss ZHE Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to a Zeiss ZHE cube.
    Full story

    zeiss cube assembly guide thumbnail

  • Zeiss Threaded Filter Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to a Zeiss Threaded Filter cube.
    Full story

    Zeiss Threaded Filter Cube Assembly Guide thumbnail

  • Nikon TE2000 Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to a Nikon TE2000 Cube.
    Full story

    nikon te2000 cube assembly guide thumbnail

  • Nikon Quadfluor Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to a Nikon Quadfluor Cube.
    Full story

    nikon quadfluor assembly guide thumbnail

  • Olympus Cube Assembly Quick How-To Guide

    by IDEX Health & Science

    Explore our step by step guide to add a Semrock optical filter set to an Olympus U-MF2 Cube and an Olympus U-MF/XL cube.
    Full story

    olympus cube assembly guide thumbnail

  • Periodic Table of Analytical Fittings Poster

    by IDEX Health & Science
    Our Periodic Table of Analytical Fittings illustrates over 100 analytical fittings’ pressure rating, tubing size, head style, fitting material, and torque required. Full story

  • Fluidics Tech Tips

    by IDEX Health & Science
    Explore our tech tips and find technical topics from Avoiding Pressure Transients to how to choose a Sample Loop. Full story

  • Microfluidic Definitions & Specs

    by IDEX Health & Science
    Learn about Microfluidic Definitions and Specifications to assist in your understanding of Microfluidic applications. Full story

  • Physics of Pixel Shift

    by IDEX Health & Science
    In multicolored fluorescence microscopy, pixel shift can cause imaging errors, which can lead to erroneous interpretation of biological data. This article highlights some key considerations in the design of optical filter sets with “zero pixel shift” (less than one pixel error) performance. Full story

  • Injectors Troubleshooting Guide

    by IDEX Health & Science
    Our Troubleshooting Guide presents common problems encountered when using manual sample injection valves. Each problem (or “symptom,”; such as spurious peaks) is described and followed by a detailed discussion of possible causes and solutions. This information has been compiled by our technical staff with 25 years of technical support experience with sample injection and fluidic technology. Full story

  • Performance, Reliability, Repeatability

    by IDEX Health & Science
    With a virtually unlimited lifetime and superior performance, Semrock filters help ensure the best possible images. They may be the easiest and least expensive way to improve the performance of your microscope when compared to the cost of upgrading cameras and objectives. Full story

  • Flatness of Dichroic Beamsplitters Affects Image Quality

    by IDEX Health & Science
    Optical filters are generally comprised of multi-layered thin-film coatings on plane, parallel glass substrates. All Semrock filters use a single substrate with coatings on one or both sides to maximize transmission and reliability. The glass substrate is not always perfectly flat before coating and the intrinsic stress of hard coatings can cause slight bending of the substrate. Fortunately, this bending has no noticeable effect on light transmitted through an optical filter at or near normal incidence. For light incident at high angles of incidence, like for a 45° dichroic beamsplitter, the bent substrate creates a slight divergence of the transmitted beam axis similar to the effect of a small wedge in the substrate. Unless the radius of curvature is very small (much less than one meter) the quality of the transmitted beam remains largely unaffected. Full story

  • Transition Width and Edge Steepness

    by IDEX Health & Science

    Transition width and edge steepness are two terms often used to describe the spectral properties of edge filters and it is important to know that these two terms are related, but not interchangeable. Transition Width is the maximum distance between the laser line (where OD > 6) and the 50% transmission point. Edge Steepness is the actual steepness measured from the OD 6 point to the 50% transmission point.
    Full story

    transition width and edge steepness for optical filter graph

  • Semrock Filters Don't Burn Out

    by IDEX Health & Science
    All Semrock filters are hard coated with sophisticated Ion Beam Sputtering (IBS) technology, resulting in patented state-of-the-art filters with extremely durable hard glass coatings on a single hard glass substrate. No absorbing glass and no lamination is necessary. The result is exceptionally durable "no burn out" fluorescence filters of the highest performance. Full story

  • Scratch Dig Inspection Process

    by IDEX Health & Science
    All Semrock filters are inspected in accordance with industry standards established for optical components. Learn how our team inspects each filter. Full story

  • Measuring Light with Wavelengths and Wavenumbers

    by IDEX Health & Science
    The "color" of light is generally identified by the distribution of power or intensity as a function of wavelength λ. Visible light has a wavelength that ranges from about 400 nm to just over 700 nm. Sometimes it is convenient to describe light in terms of units called "wavenumbers," where the wavenumber w is typically measured in units of cm-1 ("inverse centimeters") and is simply equal to the inverse of the wavelength, and is therefore proportional to frequency. Full story

  • RazorEdge Filter Layout

    by IDEX Health & Science

    In many high-performance Raman systems with microscopic imaging capabilities or highly sensitive remote probes it is desirable for the excitation laser beam and the Raman-shifted signal light to share a common light path. Illustrations in this tech note describe the two layouts and the ideal filters for each system.
    Full story