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In this article we briefly describe the method to calculate the three main
parameters that fully specify color in this system: luminosity, dominant wavelength, and
excitation purity.
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Explore our step by step guide to add a Semrock optical filter set to Leica DM-K, DM-R and DM-IRB cubes.
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Explore our step by step guide to add a Semrock optical filter set to a Zeiss ZHE cube.
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Explore our step by step guide to add a Semrock optical filter set to a Zeiss Threaded Filter cube.
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Explore our step by step guide to add a Semrock optical filter set to a Nikon TE2000 Cube.
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Explore our step by step guide to add a Semrock optical filter set to a Nikon Quadfluor Cube.
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Explore our step by step guide to add a Semrock optical filter set to an Olympus U-MF2 Cube and an Olympus U-MF/XL cube.
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Transition width and edge steepness are two terms often used to describe the spectral properties of edge filters and it is important to know that these two terms are related, but not interchangeable. Transition Width is the maximum distance between the laser line (where OD > 6) and the 50% transmission point. Edge Steepness is the actual steepness measured from the OD 6 point to the 50% transmission point.
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In many high-performance Raman systems with microscopic imaging capabilities or highly sensitive remote probes it is desirable for the excitation laser beam and the Raman-shifted signal light to share a common light path. Illustrations in this tech note describe the two layouts and the ideal filters for each system.
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