While most applications call for optical filters to be used at normal incidence, it is important to understand how the spectral properties of different types of filters change when using these filters a non-normal angles of incidence (AOI). There are two main effects exhibited by all filter spectrum as the angle is increased from normal which are discussed in this article.
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Fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then emits light at longer wavelengths within its emission band. For example, brightly fluorescent molecules (called fluorophores) can be attached to biologically significant molecules in e.g. cell membranes, in the brain, or even on subunits of DNA, whose structures become visible in a fluorescence microscope that allows us to track the way cells function in health and disease. Fluorescence is widely used in biology, biotechnology, and medicine, due to its extraordinary sensitivity, high specificity, and simplicity of usage. Learn more about what optical filters are included in a fluorescence instrument and how Semrock optical filters can optimize your application.
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Dive into the world of fluidic fittings and chromatography with our newly revised Fittings 101 Guide. Learn the fundamentals of equipment fittings and accessories, as well as some basics about liquid chromatography as an analytical technique.
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IDEX Health & Science specializes in directing fluid to where it needs to be, so you can automate your fluidic process in a simple package and form factor. Our team of experts has decades of experience in life science applications to help you avoid pitfalls across a broad range of operations and accelerate your time to market.
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This Chemical Compatibility section will allow you to either check what materials will work with the chemicals you are using or planning to use; or view what compatibility ratings are given to a material you are planning to use.
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Explore our material properties guide by selecting the material of your choice from the list provided to view properties and solvent compatibility
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Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Laser damage may be broadly classified into two types: absorption-driven and dielectric-breakdown damage. Which type dominates depends on the material properties (absorption coefficient, specific heat, melting temperature, as well as defects that cause scattering and concentrated electric field effects), geometric properties of the sample (thickness, homogeneity, surface morphology, etc.), and of course the properties of the laser beam itself.
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In this article we briefly describe the method to calculate the three main parameters that fully specify color in this system: luminosity, dominant wavelength, and excitation purity.
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Explore our step by step guide to add a Semrock optical filter set to Leica DM-K, DM-R and DM-IRB cubes.
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Explore our step by step guide to add a Semrock optical filter set to a Zeiss ZHE cube.
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Explore our step by step guide to add a Semrock optical filter set to a Zeiss Threaded Filter cube.
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Explore our step by step guide to add a Semrock optical filter set to a Nikon TE2000 Cube.
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Explore our step by step guide to add a Semrock optical filter set to a Nikon Quadfluor Cube.
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Explore our step by step guide to add a Semrock optical filter set to an Olympus U-MF2 Cube and an Olympus U-MF/XL cube.
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Our Periodic Table of Analytical Fittings illustrates over 100 analytical fittings’ pressure rating, tubing size, head style, fitting material, and torque required.
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Explore our tech tips and find technical topics from Avoiding Pressure Transients to how to choose a Sample Loop.
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In multicolored fluorescence microscopy, pixel shift can cause imaging errors, which can lead to erroneous interpretation of biological data. This article highlights some key considerations in the design of optical filter sets with “zero pixel shift” (less than one pixel error) performance.
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Our troubleshooting guide provides solutions to common problems encountered when operating our manual sample injection valves. Each problem/symptom is described and is followed by a detailed discussion of potential causes and solutions. This information is a compilation of 25 years of technical expertise and experience with sample injection and fluidic technology.
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With a virtually unlimited lifetime and superior performance, Semrock optical filters help ensure the best possible images. They may be the easiest and least expensive way to improve the performance of your microscope when compared to the cost of upgrading cameras and objectives.
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