Resource Library

Explore our step by step guide to add a Semrock optical filter set to a Nikon Quadfluor Cube. Learn More

Explore our step by step guide to add a Semrock optical filter set to an Olympus U-MF2 Cube and an Olympus U-MF/XL cube. Learn More

Our Periodic Table of Analytical Fittings illustrates over 100 analytical fittings’ pressure rating, tubing size, head style, fitting material, and torque required. Learn More

Explore our tech tips and find technical topics from Avoiding Pressure Transients to how to choose a Sample Loop. Learn More

In multicolored fluorescence microscopy, pixel shift can cause imaging errors, which can lead to erroneous interpretation of biological data. This article highlights some key considerations in the design of optical filter sets with “zero pixel shift” (less than one pixel error) performance. Learn More

Our troubleshooting guide provides solutions to common problems encountered when operating our manual sample injection valves. Each problem/symptom is described and is followed by a detailed discussion of potential causes and solutions. This information is a compilation of 25 years of technical expertise and experience with sample injection and fluidic technology. Learn More

With a virtually unlimited lifetime and superior performance, Semrock optical filters help ensure the best possible images. They may be the easiest and least expensive way to improve the performance of your microscope when compared to the cost of upgrading cameras and objectives. Learn More

Optical filters are generally comprised of multi-layered thin-film coatings on plane, parallel glass substrates. All Semrock filters use a single substrate with coatings on one or both sides to maximize transmission and reliability. The glass substrate is not always perfectly flat before coating and the intrinsic stress of hard coatings can cause slight bending of the substrate. Fortunately, this bending has no noticeable effect on light transmitted through an optical filter at or near normal incidence. For light incident at high angles of incidence, like for a 45° dichroic beamsplitter, the bent substrate creates a slight divergence of the transmitted beam axis similar to the effect of a small wedge in the substrate. Unless the radius of curvature is very small (much less than one meter) the quality of the transmitted beam remains largely unaffected. Learn More

Transition width and edge steepness are two terms often used to describe the spectral properties of edge filters and it is important to know that these two terms are related, but not interchangeable. Transition Width is the maximum distance between the laser line (where OD > 6) and the 50% transmission point. Edge Steepness is the actual steepness measured from the OD 6 point to the 50% transmission point. Learn More