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While most applications call for optical filters to be used at normal incidence, it is important to understand how the spectral properties of different types of filters change when using these filters a non-normal angles of incidence (AOI). There are two main effects exhibited by all filter spectrum as the angle is increased from normal which are discussed in this article. Learn More

Check valves are critical to HPLC pump performance, as they prevent any backflow and ensure maximum pumping efficiency. Discover critical elements related to the functional performance of a check valve and ultimately the overall chromatography results. Learn More

IDEX Health & Science specializes in directing fluid to where it needs to be, so you can automate your fluidic process in a simple package and form factor. Our team of experts has decades of experience in life science applications to help you avoid pitfalls across a broad range of operations and accelerate your time to market. Learn More

You are about to embark on an exciting journey designed to help you grasp the fundamentals of equipment fittings and accessories, as well as some basics about liquid chromatography as an analytical technique. Learn More

Fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then emits light at longer wavelengths within its emission band. For example, brightly fluorescent molecules (called fluorophores) can be attached to biologically significant molecules in e.g. cell membranes, in the brain, or even on subunits of DNA, whose structures become visible in a fluorescence microscope that allows us to track the way cells function in health and disease. Fluorescence is widely used in biology, biotechnology, and medicine, due to its extraordinary sensitivity, high specificity, and simplicity of usage. Learn more about what optical filters are included in a fluorescence instrument and how Semrock optical filters can optimize your application. Learn More

This Chemical Compatibility section will allow you to either check what materials will work with the chemicals you are using or planning to use; or view what compatibility ratings are given to a material you are planning to use. Learn More

Explore our material properties guide by selecting the material of your choice from the list provided to view properties and solvent compatibility Learn More

Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Laser damage may be broadly classified into two types: absorption-driven and dielectric-breakdown damage. Which type dominates depends on the material properties (absorption coefficient, specific heat, melting temperature, as well as defects that cause scattering and concentrated electric field effects), geometric properties of the sample (thickness, homogeneity, surface morphology, etc.), and of course the properties of the laser beam itself. Learn More

In this article we briefly describe the method to calculate the three main parameters that fully specify color in this system: luminosity, dominant wavelength, and excitation purity. Learn More

Explore our step by step guide to add a Semrock optical filter set to Leica DM-K, DM-R and DM-IRB cubes. Learn More