Educational Materials

Due to many desirable properties like high brightness, stability, longevity and narrow spectral bandwidth, lasers are replacing conventional broadband light sources for fluorescence imaging applications. Not only have these features of lasers allowed for higher-sensitivity visualization and enhanced throughput in imaging applications, but also several other unique properties of lasers—narrow beam divergence, high degree of spatial and temporal coherence, and well-defined polarization properties—have enabled new fluorescence imaging techniques. At the same time, the use of lasers as fluorescence light sources imposes new constraints on imaging systems and their components. Learn More

The term Group Delay in optics is the relative amount of time light of different frequencies is delayed as it travels through a transmitting medium (like an optic made of glass) or it rattles around inside the many layers of a thin-film coating. At some frequencies (or wavelengths), it takes light longer to emerge from the medium or thin-film coating than at other frequencies. Learn More

It is possible to increase blocking by combining two or more filters together. However, with modern high-performance filters that block light by reflection, one should not try to increase blocking merely by stacking two filters immediately next to one another—combining filters this way only works when multiple reflections between the filters are reduced or eliminated! Learn More

Despite the availability of “standard filter sets” (a combination of optimally designed exciter, dichroic and emitter filters) offered by optical filter manufacturers, it is not uncommon for researchers to build their own “custom filter sets” comprised of unique combinations of otherwise standard individual filters. Explore common pitfalls and key considerations when building custom optical filter sets. Learn More

Polymer fittings and ferrules have proven to be superior to stainless steel in many analytical applications. IDEX Health & Science has compiled the following solvent compatibility information for polymers commonly used in HPLC. Learn More

High Performance Liquid Chromatography (HPLC) is an analytical process designed to separate, quantify and analyze components of a chemical mixture. Ultra-High Performance Liquid Chromatography (UHPLC) is HPLC operated at extremely high pressure (>12,000 psi / 800 bar). Learn More

In this paper we discuss various important characteristics of hard-coated thin-film interference filters, such as high light throughput, steep edges, and high out-of-band blocking, all of which require careful consideration when designing and manufacturing optical filters for NLO imaging applications. Learn More

Optical filters play a vital role in obtaining maximum performance from complex, expensive, laser-based microscopes and it only makes sense to invest in optical filters that match the performance of the imaging system. Learn More

Get started using the Systec Vacuum Degasser with this set step-by-step quick start instructions. Learn More