Educational Materials

Multilayer optical thin-film filters play a critical role in applications ranging from biomedical and analytical instrumentation to advanced laser systems. Learn why thin-film coating technology has been a technological breakthrough in biotechnology and other fields. Learn More

Discover how the Semrock optical filters business was started back in 2000. Learn More

Multimodal nonlinear optical (NLO) imaging is a microscopy approach used in a variety of biomedical applications. It harnesses and integrates the unique capabilities of nonlinear processes such as multiphoton fluorescence, second- and third-harmonic generation (SHG and THG), and coherent Raman scattering (CRS) and combines them seamlessly into a single, unified microscopy platform. Learn More

Optical edge filters are critical to the performance of Raman detection systems. This article describes two families of long‐wave‐pass (LWP) edge filters that Semrock has developed specifically for Stokes‐shift backscattered Raman signal detection systems. These filters are used in commercial Raman systems for polymer science, thin film analysis, geoscience, and the pharmaceutical industry Learn More

Thin-film silicon (Si) photovoltaics are a combination of amorphous and microcrystalline Si; monitoring the proportions via Raman spectroscopy and high-performance optical filters allows the cells to be optimized during manufacture. Learn More

Increasingly popular, total-internal-reflection-fluorescence (TIRF) microscopy systems prefer lasers for excitation because of their spectral purity and brightness. But the use of lasers can restrict other TIRF system components. Clearing these hurdles requires some careful considerations. Learn More

594-nm laser excitation can enhance our ability to analyze cells by flow cytometry. This laser wavelength fills a significant excitation gap between the now common 561-nm line and the red laser sources. Adding this wavelength to the excitation repertoire enables excitation of virtually any visible fluorescent probe for cytometric analysis. Learn More

Filter optimization is particularly important for applications where the FP signal and percentage of positive events are relatively low. In this article discover how the results substantially improved yield of GFP-expressing parasites, and requires decreased sorting time in comparison to standard methods. Learn More

With carefully controlled absorption and scattering losses, high-reflectivity, low-loss dielectric mirrors enable demanding applications such as gravitational-wave detection and cavity ring-down spectroscopy. Learn More

Optical filters are crucial in fluorescence experiments and instrumentation. Explore recent advances in filter technology offer a way to improve fluorescence techniques in this journal article. Learn More