Optimization of Flow Cytometric Detection and Cell Sorting of Transgenic Plasmodium Parasites using Interchangeable Optical Filters

by IDEX Health & Science

Filter optimization is particularly important for applications where the FP signal and percentage of positive events are relatively low, such as analysis of parasite-infected samples with in the intention of gene-expression profiling and analysis. The approach outlined here results in substantially improved yield of GFP-expressing parasites, and requires decreased sorting time in comparison to standard methods. It is anticipated that this protocol will be useful for a wide range of applications involving rare events.

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