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Microfluidic Definitions & Specs
By IDEX Health & ScienceLearn about Microfluidic Definitions and Specifications to assist in your understanding of Microfluidic applications. -
Physics of Pixel Shift
By IDEX Health & ScienceIn multicolored fluorescence microscopy, pixel shift can cause imaging errors, which can lead to erroneous interpretation of biological data. This article highlights some key considerations in the design of optical filter sets with “zero pixel shift” (less than one pixel error) performance. -
Injectors Troubleshooting Guide
By IDEX Health & ScienceOur Troubleshooting Guide presents common problems encountered when using manual sample injection valves. Each problem (or “symptom,”; such as spurious peaks) is described and followed by a detailed discussion of possible causes and solutions. This information has been compiled by our technical staff with 25 years of technical support experience with sample injection and fluidic technology. -
Performance, Reliability, Repeatability
By IDEX Health & ScienceWith a virtually unlimited lifetime and superior performance, Semrock filters help ensure the best possible images. They may be the easiest and least expensive way to improve the performance of your microscope when compared to the cost of upgrading cameras and objectives. -
Flatness of Dichroic Beamsplitters Affects Image Quality
By IDEX Health & ScienceOptical filters are generally comprised of multi-layered thin-film coatings on plane, parallel glass substrates. All Semrock filters use a single substrate with coatings on one or both sides to maximize transmission and reliability. The glass substrate is not always perfectly flat before coating and the intrinsic stress of hard coatings can cause slight bending of the substrate. Fortunately, this bending has no noticeable effect on light transmitted through an optical filter at or near normal incidence. For light incident at high angles of incidence, like for a 45° dichroic beamsplitter, the bent substrate creates a slight divergence of the transmitted beam axis similar to the effect of a small wedge in the substrate. Unless the radius of curvature is very small (much less than one meter) the quality of the transmitted beam remains largely unaffected. -
Transition Width and Edge Steepness
By IDEX Health & ScienceTransition width and edge steepness are two terms often used to describe the spectral properties of edge filters and it is important to know that these two terms are related, but not interchangeable. Transition Width is the maximum distance between the laser line (where OD > 6) and the 50% transmission point. Edge Steepness is the actual steepness measured from the OD 6 point to the 50% transmission point. Learn More
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Semrock Filters Don't Burn Out
By IDEX Health & ScienceAll Semrock filters are hard coated with sophisticated Ion Beam Sputtering (IBS) technology, resulting in patented state-of-the-art filters with extremely durable hard glass coatings on a single hard glass substrate. No absorbing glass and no lamination is necessary. The result is exceptionally durable "no burn out" fluorescence filters of the highest performance. -
Scratch Dig Inspection Process
By IDEX Health & ScienceAll Semrock filters are inspected in accordance with industry standards established for optical components. Learn how our team inspects each filter. -
Measuring Light with Wavelengths and Wavenumbers
By IDEX Health & ScienceThe "color" of light is generally identified by the distribution of power or intensity as a function of wavelength λ. Visible light has a wavelength that ranges from about 400 nm to just over 700 nm. Sometimes it is convenient to describe light in terms of units called "wavenumbers," where the wavenumber w is typically measured in units of cm-1 ("inverse centimeters") and is simply equal to the inverse of the wavelength, and is therefore proportional to frequency. -
RazorEdge Filter Layout
By IDEX Health & ScienceIn many high-performance Raman systems with microscopic imaging capabilities or highly sensitive remote probes it is desirable for the excitation laser beam and the Raman-shifted signal light to share a common light path. Illustrations in this tech note describe the two layouts and the ideal filters for each system. Learn More
