Educational Materials

You are about to embark on an exciting journey designed to help you grasp the fundamentals of equipment fittings and accessories, as well as some basics about liquid chromatography as an analytical technique. You have 2 choices. We have a digital PDF file available for you to download now below or you can request a printed poster by filling out the form. Learn More

Introduction to Fluorescence Filters

Optical fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then nearly instantaneously emits light at longer wavelengths within its emission band. For analytical purposes, strongly fluorescing molecules known as fluorophores are attached to biological molecules and other targets of interest to enable identification, quantification, and even real-time observation of biological and chemical activity. Fluorescence is widely used in biotechnology and analytical applications due to its extraordinary sensitivity, high specificity, and simplicity.

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Chemical Compatibility

This Chemical Compatibility section will allow you to either check what materials will work with the chemicals you are using or planning to use; or view what compatibility ratings are given to a material you are planning to use.

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Materials Guide

Explore our material properties guide by selecting the material of your choice from the list provided to view properties and solvent compatibility

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Laser Damage Threshold

Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Laser damage may be broadly classified into two types: absorption-driven and dielectric-breakdown damage. Which type dominates depends on the material properties (absorption coefficient, specific heat, melting temperature, as well as defects that cause scattering and concentrated electric field effects), geometric properties of the sample (thickness, homogeneity, surface morphology, etc.), and of course the properties of the laser beam itself.

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