Fluorescence Filter Information

by IDEX Health & Science

Need basic information on fluorescence filter applications and uses? Find tech notes and white papers about this filter type in the section below.


Introduction to Fluorescence Filters

Optical fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then nearly instantaneously emits light at longer wavelengths within its emission band.

Multiband Filter Set Terminology

To satisfy the ever-increasing demand for high-speed imaging, especially for live-cell real-time analysis using fluorescent protein labels, there is a need for an alternative to the single-band filter cube approach without sacrificing image fidelity.

Fluorescent Proteins Theory Applications and Best Practices

In this white paper, the history and development of fluorescent proteins is discussed, along with what they are and how they work. Applications of fluorescent proteins are covered, as are considerations for application success.

Choosing Dichroic Beamsplitters with Flatness/ RWE Appropriate to the Microscopy Method

Wavefront distortion can degrade image quality by reducing contrast or compromising resolution. This article explains how to select optical filters for high performance microscopy, and provides guidance on choosing Semrock catalog filters for wavefront distortion performance required for applications.

Super-Resolution Dichroic Beamsplitters

Semrock optical filters for super-resolution microscopy with λ/5 P-V RWE on 3 mm thick dichroics and 1λ P-V RWE on 1 mm dichroics. These industry leading optics improve the performance of laser based confocal and TIRF illumination systems, and are ideal for reflection of imaging beams in conventional structured-illumination techniques as well as patterned illumination systems for localized photo-activation.

Image-splitting Dichroic Beamsplitter

The spectral edges of these filters are optimized for imaging of popular fluorophore-pairs providing maximum signal throughput, while maintaining minimal wavefront distortion in reflection and transmission thereby maximizing contrast and resolution of the overall imaging system.

Super-Resolution Microscopy Cubes

Super-resolution microscopy cubes set the standard for laser based microscopes. These cubes are optimized for mounting 1λ RWE 1mm thick super-resolution laser dichroic beamsplitters. Maximize SNR and minimize artifacts in TIRF, Confocal, PALM, STORM, SIM, and other super-resolution techniques.

Flatness of Dichroic Beamsplitters

Glass substrate is not always perfectly flat, especially after it is coated, since the intrinsic stress of hard glass coatings can cause slight bending of the substrate. This bending can cause focal plane shift and image distortion when imaging reflected light.

Practical Flatness

BrightLine® laser dichroic beamsplitters set a new standard for super-resolution microscopy with λ/5 P-V Reflected Wavefront Error (RWE) performance. We’ll explain how dichroic beamsplitter flatness affects an optical wavefront in reflection, and how to calculate the practical impact of flatness specifications on your system.

VersaChrome Edge tunable filters

Unlock virtually unlimited spectral flexibility for fluorescence microscopy and hyperspectral imaging, as well as for spectroscopy applications allowing users to create a bandpass filter as narrow as sub 5nm FWHM or as wide as 12% of the center wavelength throughout the visible and near-infrared wavelength ranges.

Fluorescence Resonance Energy Transfer

Fluorescence Resonance Energy Transfer (FRET) is a powerful technique for characterizing distance-dependent interactions on a molecular scale. Learn why Semrock fluorescence filters are optimal for FRET.

Ultraviolet Fluorescence

Many biological molecules of interest naturally fluoresce when excited by shorter- wavelength UV light. Because the fluorescence is intrinsic, samples can be observed without the added chemistry and limitations associated with "indirect" labeling by extrinsic fluorophores.

Using Fura-2 to Track Ca2+

The fluorophore Fura-2 has an absorption spectrum that varies markedly depending on the concentration of calcium (Ca2+) that is present near the fluorophore molecule.

BrightLine® Multiphoton LaserMUX Beam Combiner

Multiphoton LaserMUX beam combiners enable deeper tissue imaging and improved contrast in multi-color and multi-modal fluorescence microscopy