LED-based Light Engine Filter Sets for Fluorescence Imaging
BrightLine® LED-based Light Engine Filter Sets
LED-based light engines are leading the way for fluorescence imaging. However, the full potential of LED light engines remains to be realized in many imaging configurations because they are still being used with conventional filter sets designed for mercury or xenon arc lamps.
These legacy broadband light sources have a significantly different spectral signature and energy distribution compared to the newer LED sources. Therefore conventional filter sets may not provide optimal performance with the new LED sources. Semrock’s LED-based light engine filter sets are aligned to the unique spectral peaks of the most popular LED-based light engines on the market today. These filter sets simultaneously deliver the brightest signal and the highest signal-to-noise ratio (contrast) available for imaging a range of fluorophores.
Semrock’s LED-based light engine filter sets are also designed to use common excitation filters between single-band and corresponding multiband configurations, simplifying interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between single-band and multiband filter sets, but also provides significant cost savings.
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Benefits of BrightLine® LED Filter Sets
- 10s to 100s of percent more signal per channel compared to filter sets designed for conventional light sources
- Common single-band excitation filters across the family of LED sets simplifies switching between corresponding multiband and single-band sets by only needing to replace dichroic and emitter
- Only filter sets in the market today that are spectrally aligned to popular LED-based light engines from CoolLED, Excelitas Technologies (X-Cite®), and Lumencor
- Available in single-band, Full, Pinkel and Sedat multiband filter set configurations
- Unravel the full potential of your LED-based light engines by experiencing optimized filtering from Semrock’s LED filter sets
| BrightLine® Single-band LED Filter Sets | |||
| Filter Set / Primary Fluorophores | Excitation (CWL/BW) | Emission (CWL/BW) | Dichroic (Edge) |
| LED-DAPI-A DAPI, Alexa Fluor® 405, BFP, Pacific Blue™ | 392/23 | 447/60 | 409 nm |
| LED-CFP-A CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | 438/24 | 483/32 | 458 nm |
| LED-FITC-A FITC (Fluorescein), GFP (EGFP), Cy2™, 5-FAM | 474/27 | 525/45 | 495 nm |
| LED-YFP-A YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | 509/22 | 544/24 | 526 nm |
| LED-TRITC-A TRITC, SpectrumOrange, dTomato, mTangerine | 554/23 | 609/54 | 573 nm |
| LED-mCherry-A mCherry, TexasRed, mKate, mRFP1 | 578/21 | 641/75 | 596 nm |
| LED-Cy5-A Cy5™, Alexa Fluor® 647,APC | 635/18 | 680/42 | 652 nm |
| BrightLine® Multiband LED Filter Sets – single or multiband exciters, single or multiband emitters, and multiedge beamsplitter | |||
| Filter Set / Primary Fluorophores | Excitation (CWL/BW) | Emission (CWL/BW) | Dichroic (Edge) |
| LED-DA/FI/TR/Cy5-A (Full Multiband) Blue: DAPI, BFP, Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5™, APC, Alexa Fluor® 647 | 392/23 474/27 554/23 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm |
| LED-DA/FI/TX-3X-A (Triple-band Pinkel Multiband) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Cy2™ Red: Texas Red, mCherry 5-ROX | Ex1: 392/23 Ex2: 474/27 Ex3: 575/25 | 432/36 523/46 702/196 | 409 nm 493 nm 596 nm |
| LED-CFP/YFP/mCherry-3X-A (Triple-band Pinkel Multiband) Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP Yellow: YFP (EYFP), Venus, Fluo-3, Rhodamine 123 Red: mCherry, TexasRed, mKate, mRFP1 | Ex1: 438/24 Ex2: 509/22 Ex3: 578/21 | 475/22 543/22 702/197 | 459 nm 526 nm 596 nm |
| LED-DA/FI/TR/Cy5-4X-A (Quad-band Pinkel Multiband) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5™, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm |
| LED-DA/FI/TX-3X3M-A (Triple-band Sedat Multiband) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Cy2™ Red: TexasRed, mCherry 5-ROX | Ex1: 392/23 Ex2: 474/27 Ex3: 575/25 | Em1: 433/24 Em2: 525/45 Em3: 641/75 | 409 nm 493 nm 596 nm |
| LED-CFP/YFP/mCherry-3X3M-A (Triple-band Sedat Multiband) Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP Yellow: YFP (EYFP), Eosin, Fluo-3, Rhodamine 123 Red: mCherry, TexasRed, mKate, mRFP1 | Ex1: 438/24 Ex2: 509/22 Ex3: 578/21 | Em1: 482/25 Em2: 544/24 Em3: 641/75 | 459 nm 526 nm 596 nm |
| LED-DA/FI/TR/Cy5-4X4M-A (Quad-band Sedat Multiband) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5™, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | Em1: 433/24 Em2: 520/35 Em3: 600/37 Em4: 680/42 | 409 nm 493 nm 573 nm 652 nm |