Educational Materials

There are three types of multiband filter sets for simultaneous multi-color imaging -- the "full-multiband", the "Pinkel", and the "Sedat". Learn More

In this article our aim is to answer some basic questions about the polarization of light, including: what polarization is and how it is described, how it is controlled by optical components, and when it matters in optical systems. Learn More

Super-resolution microscopy allows for the visualization of cellular samples with a resolution similar to that of an electron microscope, yet it retains the advantages of an optical fluorescence microscope. Discover key recent developments in super-resolution microscopy in this white paper. Learn More

Given the critical role optical filters play in fluorescence microscopy, it is important to understand how such filters transmit both the desired fluorescence signal as well as the undesired background light. Learn more about spectral modeling in fluorescence microscopy in this white paper. Learn More

This article outlines a novel approach to spectral imaging based on recently introduced tunable thin-film optical filters. Learn More

VersaChrome has now made possible thin-film bandpass and edge filters that are tunable over a wide range of wavelengths by adjusting the angle of incidence with essentially no change in spectral performance. As a result, many optical systems can now benefit from tunable filters with the spectral and two-dimensional imaging performance characteristics of thin-film filters and the center wavelength tuning speed and flexibility of a diffraction grating. Learn More

Multicolor imaging in fluorescence microscopy is typically performed by sequentially acquiring images of different colors. An overlay of these images is used to study the relative spatial distribution of various types of cellular components. However, in order to ensure that such a composite image is a true representation of the biological phenomena under investigation, it is important to understand imaging artifacts such as “pixel shift” error in multicolor fluorescence imaging. Learn More

In this article, we discuss the emergence of NLO imaging and how it has been facilitated by advances in three key technology areas: ultrafast lasers; high-performance, hard-coated optical filters; and high-sensitivity detectors. Fluorophores commonly used in combination with NLO fluorescence imaging will also be discussed. Learn More

Even though fluorescence microscopy has become a routine technique for many applications, demanding requirements from technological advances continue to push the limits. Lack of sufficient flatness on a dichroic is not only a problem for imaging quality, but it can also reduce the quality of the illumination light beam when the dichroic is used to reflect the illumination light toward the sample. Learn More

This article compares the strengths and weaknesses of some of the most popular component solutions on the market today, and then explains how Semrock’s advanced optical filter technology is being applied to a new class of polarization components designed specifically for lasers. This new class of filters is filling some key gaps in the polarization component market. Learn More