Fluidics FAQs

Here we have provided comprehensive lists of commonly asked questions regarding our fluidics products and related applications. This information is designed to support your inquiries, but if you don’t find the answers you are looking for we encourage you to contact us for further assistance.

Please use the sort buttons in the left navigation to navigate between questions and answers that are specific to our Optical Filters, Optical Imaging Systems, Cameras, Microfluidics, Webstore, and Engineering Partnership.

What is Sparging?

Sparging is the process of bubbling a virtually insoluble gas, such as helium, through a solvent in an effort to displace the gases which have dissolved into solution. A gas such as helium has a very low solubility in most solvents used in HPLC. By forcing an inert gas like helium into a solvent which doesn’t “want” the gas to be present, the gases (like oxygen and nitrogen) that are present are driven out (Henry’s Law). This leaves a solvent with virtually no gas dissolved in solution. Therefore, when the gradient mixing takes place, bubble formation is minimized, helping to ensure better chromatographic results.