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There are two very common sizes of tubing you will find in HPLC. The most common size of tubing is 1/16″ outer diameter, or OD. You can usually find stainless steel and PEEK tubing of this size as part of your system. You might also find PFA or Tefzel® tubing of this size.
The other most common size of HPLC tubing is 1/8″ OD. Usually tubing of this size is used as solvent transfer tubing from the solvent reservoir to the inlet side of the pump. Most often, it is manufactured from Teflon.
There are other sizes of tubing you might find in an HPLC system, ranging from microbore tubing, with ODs that range from .004″ up to 1/16″, to larger 3/16″ OD tubing and even 1/4″ OD tubing.