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A sample is separated into its components due to a process termed differential migration. This migration is controlled by the degree of attraction each component has for the stationary phase compared to the mobile phase. Usually, the various components that make up a sample will exhibit varied degrees of attraction, and it is based on these varied levels of attraction that separation takes place.
Optimal separations can be achieved by making strength adjustments to the stationary and/or mobile phases and selecting the right chemistries for these two phases can enhance the differential migration of the sample components.