
Semrock utilizes Vacuum-Release (VR) Gel-Pak packaging for filters whose part size and narrow substrate thickness make it difficult to remove the filters from standard Gel-Paks. Learn More
While most applications call for optical filters to be used at normal incidence, it is important to understand how the spectral properties of different types of filters change when using these filters a non-normal angles of incidence (AOI). There are two main effects exhibited by all filter spectrum as the angle is increased from normal which are discussed in this article. Learn More
You are about to embark on an exciting journey designed to help you grasp the fundamentals of equipment fittings and accessories, as well as some basics about liquid chromatography as an analytical technique. Learn More
Fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then emits light at longer wavelengths within its emission band. For example, brightly fluorescent molecules (called fluorophores) can be attached to biologically significant molecules in e.g. cell membranes, in the brain, or even on subunits of DNA, whose structures become visible in a fluorescence microscope that allows us to track the way cells function in health and disease. Fluorescence is widely used in biology, biotechnology, and medicine, due to its extraordinary sensitivity, high specificity, and simplicity of usage. Learn more about what optical filters are included in a fluorescence instrument and how Semrock optical filters can optimize your application. Learn More
This Chemical Compatibility section will allow you to either check what materials will work with the chemicals you are using or planning to use; or view what compatibility ratings are given to a material you are planning to use. Learn More
Explore our material properties guide by selecting the material of your choice from the list provided to view properties and solvent compatibility Learn More
Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Laser damage may be broadly classified into two types: absorption-driven and dielectric-breakdown damage. Which type dominates depends on the material properties (absorption coefficient, specific heat, melting temperature, as well as defects that cause scattering and concentrated electric field effects), geometric properties of the sample (thickness, homogeneity, surface morphology, etc.), and of course the properties of the laser beam itself. Learn More
Our Periodic Table of Analytical Fittings illustrates over 100 analytical fittings’ pressure rating, tubing size, head style, fitting material, and torque required. Learn More
Explore our tech tips and find technical topics from Avoiding Pressure Transients to how to choose a Sample Loop. Learn More
Our Troubleshooting Guide presents common problems encountered when using manual sample injection valves. Each problem (or “symptom,”; such as spurious peaks) is described and followed by a detailed discussion of possible causes and solutions. This information has been compiled by our technical staff with 25 years of technical support experience with sample injection and fluidic technology. Learn More