Stories & Features

Welcome to Semrock’s SearchLight™ Spectral Plotting & Analysis Tool

Aug 3, 2018 by IDEX Health & Science

The Premier Modeling Resource for Fluorescence Microscopists and Optical System Designer

Start Using SearchLight

SearchLight™ is Semrock’s free, online spectral plotting and analysis tool that allows fluorescence microscope users and optical instrument designers to model and evaluate the spectral performance of fluorophores, filter sets, light sources, and detectors as components of an overall system. Its intuitive user interface makes it easy to manipulate spectral component specifications and plot spectral results. SearchLight’s built-in System Calculator allows the user to quantify system metrics including signal, SNR, and bleed-through; as well as compare various system configurations against each other.

Any number of spectra may be plotted simultaneously, and an entire graphing session sent to colleagues for collaboration using the “Share” feature. SearchLight sessions can also be saved into your personal account for future use.

SearchLight contains an extensive library of over 800+ fluorophores, 150+ light sources, more than a dozen detectors, and Semrock’s entire library of filters and filter sets. “MyData” allows you to upload your own custom datasets for any components not currently included as a selection choice.

  • Evaluate and optimize your system configurations
  • Save, Share, & Export your results for better collaboration
  • Take advantage of the regularly updated databases
  • Save time in the lab and make every photon count with SearchLight

Intelligent User Experience within SearchLight

  • Find compatible products for a selected fluorophore
  • Narrow list of potential exciters, emitters, dichroics, or filter sets

SearchLight was one of the featured tech products on NewsWatchTV airing on January 6th, 2017. NewswatchTV airs on the Discovery Channel, AMC, and ION Network in over 200 Markets nationwide in the US. Enjoy the following feature.


Compare System Calculator

Our SearchLight Compare System Calculator feature quantitatively computes the fluorescence signal, noise, and signal-to-noise ratio for unlimited combinations of filter sets, fluorophores, light sources, and detectors. Calculations are based on a straightforward mathematical framework, which is described in detail in our white paper Spectral Modeling in Fluorescence Microscopy.

SearchLight Compare System Calculator takes into account all the spectral components of a typical epifluorescence microscope configuration. See our Spectral Modeling in Fluorescence Microscopy white paper for additional details.