Stories & Features

Welcome to Semrock’s SearchLight™ Spectral Plotting & Analysis Tool

Aug 15, 2023 by IDEX Health & Science

The Premier Modeling Resource for Fluorescence Microscopists and Optical System Designer

Start Using SearchLight icon

To overcome the obstacles many fluorescence instrument designers and users face with spectral management, our experts created SearchLight: a free, intuitive, online plotting tool that gives you the power to optimize your fluorescence system.

woman with laptop has SearchLight software on the screen

SearchLight allows fluorescence microscope users and optical instrument designers to model and evaluate the spectral performance of fluorophores, filter sets, light sources, and detectors as components of an overall system. Its intuitive user interface makes it easy to manipulate component specifications and plot spectral results that allow users to make smarter decisions without wasting time and money.

  • Expansive Resources: Utilize SearchLight’s intuitive interface containing the world’s most expansive component database 
  • Advanced Features: Streamline your design process by easily selecting elements, visualizing properties, and quickly calculating critical parameters such as signal brightness and signal-to-noise ratio
  • Collaborate with Peers: Easily upload your own filter or fluorophore data and share it with colleagues to evaluate and edit at no cost.
  • Protect Your Data: SearchLight is not a cloud-based software and allows you to maintain full control of your privacy settings

NEW IN 2023

  • New Spectra added per customer requests
  • 40+ New Filters added 
  • 10 Fluorophores added

example of a spectra on SearchLight tool

SearchLight’s built-in System Calculator also allows the user to quantify system metrics including signal, SNR, and bleed-through; as well as compare various system configurations against each other.

Any number of spectra may be plotted simultaneously, and an entire graphing session sent to colleagues for collaboration using the “Share” feature. SearchLight sessions can also be saved into your personal account for future use.

SearchLight contains an extensive library of over 800+ fluorophores, 150+ light sources, more than a dozen detectors, and Semrock’s entire library of filters and filter sets. “MyData” allows you to upload your own custom datasets for any components not currently included as a selection choice. You maintain full ownership of your data, and it will not become part of the standard options.

  • Evaluate and optimize your system configurations
  • Save, Share, & Export your results for better collaboration
  • Take advantage of the regularly updated databases
  • Save time in the lab and make every photon count with SearchLight

SearchLight search bar

Intelligent User Experience within SearchLight

  • Find compatible products for a selected fluorophore
  • Narrow list of potential exciters, emitters, dichroics, or filter sets

SearchLight Media Spotlight

Learn more about SearchLight by watching this introductory video below:

SearchLight was also one of the featured tech products on NewsWatchTV airing on January 6th, 2017. NewswatchTV airs on the Discovery Channel, AMC, and ION Network in over 200 Markets nationwide in the US. Click to watch the full segment.

Compare System Calculator

Our SearchLight Compare System Calculator feature quantitatively computes the fluorescence signal, noise, and signal-to-noise ratio for unlimited combinations of filter sets, fluorophores, light sources, and detectors. Calculations are based on a straightforward mathematical framework, which is described in detail in our white paper Spectral Modeling in Fluorescence Microscopy.

example of how SearchLight's Compare System Calculator reviews the spectral components of a typical epifluorescence microscope configuration

SearchLight Compare System Calculator takes into account all the spectral components of a typical epifluorescence microscope configuration.