WEBINAR: What is Your Photon Worth? How to Select Optimal Optical Filters

Webinar Info:

Fluorescence-based assays depend on detecting photons. Common to such assays is the notion of the ‘good’ photon, which carries useful assay information, and the ‘bad’ photon, which carries noise, background, or other information that imperils the assay. A robust assay can succeed despite some level of these ‘bad’ photons, but a more sensitive or difficult one might need a much higher ratio of ‘good’ to ‘bad’ photons to ensure success.

High performance costs more, but is more selective for ‘good’ photons, and similarly for moderate performance at lower price. In this way, a photon can be assigned a “worth” depending on its level of criticality.

In this webinar, we'll show you how to conduct a cost-benefit analysis that relates this "worth" to your system performance. We use SearchLight™, our tool for modeling photon flow in fluorescence-based optical systems, in real-world flow cytometry and fluorescence microscopy scenarios. By comparing filter sets, we reveal which filters have the best combination of price and your need for low-noise, low-crosstalk, “good” photon performance. Learn how to optimize filter selection to meet your performance requirements at your budget.

Our speaker Michael Delay, PhD explores this topic in our latest webinar. Watch the replay below!

Speaker Info:

Michael Delay, PhD has a background in experimental particle physics with a Ph.D. from the University of Washington. His subsequent work involved novel optical methods in biophysics research on cells and tissues at UCLA and the University of Calgary. He transitioned to Axon Instruments, where he was involved in developing new optics-based tools for cell biology research, and is at present with IDEX Health & Science | Semrock as a Senior Application Scientist. He continues to collaborate with researchers on topics in biology and is fascinated by the history of languages.

Webinar Replay: