Stories & Features
Step through the Spectrum with Nanopede™ Bandpass Optical Filters
Oct 13, 2023
by
IDEX Health & Science
Flow cytometry interrogates cells through scattered light measurements and fluorescent markers. In spectral flow cytometry, the fluorescence is collected across the entire spectrum using discrete, back-to-back (spectrally adjacent) optical filters. Then the photons are binned such that spectral unmixing can resolve which fluorescent markers are present in the interrogated cell. As such, spectral flow cytometry requires filters covering the UV, visible, and NIR in discrete steps, which may lead to a desire to balance instrument cost and optical filter performance.
Shop the Nanopede Filter Family
IDEX Health and Science understands these needs, and we are proud to announce our latest Semrock bandpass optical filters, which cover the near UV and visible spectrum in 20 nm Full-Width, Half-Max (FWHM) steps. Moving into the NIR, the FWHM increases to 30 nm. The twenty-six filters in the Nanopede family have been designed with your application in mind:
- Coverage of the spectrum in discrete steps using uniform specifications so you can focus on flow cytometry innovation.
- ODavg > 5 blocking from 330 to 900 nm, so you can optimize for the blocking depth and range you need.
- ODabs > 5 at key laser wavelengths to assist in reducing light source bleedthrough.
- Hard coated for consistent performance without degradation throughout your flow cytometer’s entire life span in addition to intra-instrument reliability for your most demanding applications.
- Availability in 12.5 mm housed standard sized filters with 10 mm clear aperture, providing a compact filter package. (Custom sizing is also available to fit your unique instrument footprint.)
- Available now to support your immediate prototyping needs through future volume production requirements.
The Semrock Nanopede bandpass optical filter family provides a value approach to optical performance for your application. Nanopede is just the beginning of our evolving flow cytometry product line to match the rapidly developing flow cytometry market, as well as additional detector-based applications. Our understanding of filter performance for flow cytometry and detector-based applications is why we offer a filter designed to the exact specification required.
We understand each flow cytometry instrument is different; partner with us on custom optical filters to meet your specific application needs.
Figure 1: The Nanopede bandpass optical filter family offers T > 93%. A close-up of FBP01-620/14 with its two neighboring filters is on the right.
Figure 2: The Nanopede bandpass optical filter family offers OD5 blocking. A close-up of FBP01-620/14 with its two neighboring filters is on the right.