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Historically, when scientists and clinicians looked through a traditional microscope, they saw only a thin 2D plane of a sample. This was unhelpful for understanding complex cellular processes or the relationships that various systems had to each other. While it was possible to cast various wavelengths of light deep into brain tissue, for example, researchers had to contend with the shallow and deep elements within the tissue contrasting and causing blur. So it is welcome news that a team of researchers from Boston University developed a technique that can simultaneously acquire images at various depths using a standard microscope. The method is based on a z-splitter prism. The prism splits the light into several paths, with increasing…READ MORE