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Researchers from the University of Bordeaux have found a way to calibrate stimulated emission depletion (STED) microscopy for improved brain imaging. The method increases the depth at which STED microscopy can image and be applied to other tissues.
STED microscopy requires the target sample to contain fluorophores — compounds that absorb light at one wavelength and then reemit it at a longer one. In the simplest version of the technique, fluorophores are excited in a circular spot by irradiation with a diffraction-limited focused laser. Then, a doughnut-shaped portion around the spot is irradiated with less-energetic light, called the depletion beam, that switches off the fluorescence by the process of stimulated emission. The…READ MORE