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A South Korean research team has developed an optical microscope capable of maintaining spatial resolution and acquiring a microscopic “map” of neural network activity in brain tissue as it images through the width of an intact mouse skull. The device pairs hardware components with computational adaptive optics (AO); the latter was initially conceptualized to correct optical aberrations in ground-based astronomy.
Testing showed the ability of the microscope to capture two-photon fluorescence images of a dendritic spine of a neuron behind a mouse skull with a spatial resolution near the diffraction limit. The achievement, team members report, makes it possible to examine a mouse brain in its native states.
To image a…READ MORE