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In 1942, phase microscopy revolutionized biological imaging because it enabled clear visualization of cells and their internal contents without labels or stains. This empowered biologists to study these transparent, living objects over long periods without killing them or altering their function or dynamic behavior1,2. Today, quantitative phase imaging (QPI), an extension of phase microscopy, is a very active area of research that has led to many advancements in biology, including quantification of cellular structures below a nanometer, the characterization of cell mass transport and minute cell membrane fluctuations, detailed tomographic visualization of the refractive index distribution of cells, disease characterization, and even…READ MORE