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Using 3D optical diffraction tomography (ODT), scientists at KAIST (formerly the Korea Advanced Institute of Science and Technology) gained insight into how the biodegradable polyester polyhydroxyalkanoate (PHA) accumulates in the cells of living bacteria. PHA is considered a sustainable and ecofriendly substitute to existing synthetic plastics.
Increased understanding of how PHA is formed, and of its characteristics, could further its use as an alternative to petroleum-based plastics.
The researchers performed 3D, quantitative, label-free analysis of PHA granules in individual, live bacterial cells by measuring the refractive index distributions using ODT. The approach allowed them to visualize the cells in 3D, perform a…READ MORE