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While multiphoton excitation is now firmly established as a front-line tool in microscopy, it is still an evolving field producing a steady stream of new applications and techniques, all supported by advancements in lasers and microscopes.
A key trend is the push for deeper imaging in vivo. It has recently been shown that a broadly tunable laser source enables researchers to use a “guide star” method, originally used in the field of astronomy, to perform the wavefront correction needed for high-resolution two-photon images deep in the mouse brain. Furthermore, researchers have discovered that three-photon excitation, using a new generation of amplified sources, enables deep imaging in dense tissue to help investigate the…READ MORE