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A 3D reconstruction method, developed by researchers at the ImmunoSensation2 Cluster of Excellence at the University of Bonn, the University Hospital, and the Center for Advanced European Studies and Research (caesar), uses multifocal imaging (MFI) devices to provide precise, label-free tracking of rapid biological processes in 3D. The tool allows scientists to use MFI to reconstruct the movement of quickly moving biological processes in 3D while maintaining a large field of view (FOV) and high spatiotemporal resolution.
Standard MFI devices allow high-speed 3D imaging, but they require bright fluorescent labels and are limited by the need to compromise between high resolution and large FOV.
An MFI device, when placed into the…READ MORE