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Researchers from the University of Tokyo have developed a way to increase the extent and types of information they are able to ascertain about the insides of living cells by using existing microscopy techniques. Their method does not require staining or fluorescent dyes.
To effectively image individual cells, microscope cameras must detect subtle differences in the light passing through parts of the cell, which is itself nearly translucent. Those differences indicate light’s phase. Image sensors are limited by the amount of light phase they can detect. This is referred to as dynamic range.
“To see greater detail using the same image sensor, we must expand the dynamic range so that we can detect smaller phase changes…READ MORE