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Researchers at Boston University introduced a multifocus optical microscopy technique for simultaneously acquiring images at differing depths. The method can be added to existing camera-based microscopy techniques, such as fluorescent, phase contrast, and dark-field imaging.
The development will allow users to observe living cells and organisms at high speeds and with high contrast.
Using a z-splitter prism that the research team led by Jerome Mertz assembled from off-the-shelf components, including partial beamsplitters and right-angle prisms, the researchers divided detected light to produce multiple images simultaneously inside the boundaries of a single camera frame. The team built the prism by cementing individual…READ MORE