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    Light Sheet Microscopy: Transforming 3D Fluorescence Imaging

    Article obtained from BioPhotonics RSS Feed.

    Light sheet fluorescence microscopy (LSFM) is a fast and efficient imaging technique that combines the speed of wide-field imaging with optical sectioning and low photobleaching. LSFM has become an important fluorescence imaging modality, especially for volumetric (3D) imaging. Prominent applications include developmental biology, cleared tissue imaging, cell biology, and neuroscience.

    Selective plane illumination microscopy (SPIM) initially referred to a specific form of LSFM, but SPIM has become synonymous with LSFM. “Light sheet microscopy” is also a synonym because nearly all light sheet imaging utilizes fluorescence. The terms LSFM, SPIM, and light sheet microscopy will be used interchangeably here.

    The…

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    Apr, 08 2020 |

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