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Researchers at the University of Chicago have designed a multidimensional imaging analysis pipeline for lattice light-sheet microscopy (LLSM). They set out to study T-cell function using high-dimensional microscopy, but then identified the need for an effective method of analysis.
Because of limited data points for LLSM data, the researchers did not have an effective way to analyze the data in 4D. To increase the amount of data points and allow more sophisticated analyses, researchers Jillian Rosenberg and Guoshuai Cao developed a way to treat each molecule, rather than each cell, as a data point.
They developed a pipeline composed of publicly available software packages, called lattice light-sheet microscopy multidimensional…READ MORE