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Superresolution has been a hot topic within the microscopy community since the 2014 Nobel Prize in chemistry was awarded. Breaking the diffraction limit is no small task. Despite efforts to simplify and perfect the process, it is well known that superresolution is difficult to perform in practice, and even more so with live samples. This is why it’s important to get the most out of live cell experiments by looking beyond methods and techniques.
There are many common superresolution microscopy techniques offering various benefits. From localization techniques such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), to structured illumination microscopy (SIM) and stimulated…READ MORE