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Every day, the neuronal connections within our brains change depending on what we learn and experience in our daily lives. Specialized proteins, called activity-dependent transcription factors, activate genes within the cells of the brain to help it translate this rapid incoming signaling into lasting neuronal changes. Until now, it has not been possible to directly monitor transcription factors activity.
Scientists at the Max Planck Florida Institute for Neuroscience (MPFI) have designed and developed novel biosensors that will allow the simultaneous, in vivo study of both sensory-evoked neuronal activity and transcription factor dynamics. The biosensors couple the specialized techniques of two-photon calcium imaging with two-photon…READ MORE