Improving Resolution for Size-Exclusion Chromatography Methods by Optimization - IDEX Health & ScienceIDEX Health & Science

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    Improving Resolution for Size-Exclusion Chromatography Methods by Optimization

    Article obtained from LCGC Liquid Chromatography (LC/HPLC) RSS Feed.

    Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is unique in that lower flow rates often yield improvements in separation, and resolution can improve considerably simply by running at a lower linear velocity. This application note demonstrates this approach for the aggregate analysis of NIST mAb, a common standard protein.

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    Sep, 03 2021 |

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