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A collaboration between researchers at the University of Warsaw and the Weizmann Institute of Science yielded a method of fluorescence microscopy that, in theory, has no resolution limit. In practice, the team demonstrated a fourfold improvement over the diffraction limit.
The technique, superresolution optical fluctuation image scanning microscopy (SOFISM), uses the naturally occurring fluctuations in emission intensity of fluorescent markers to further enhance the image scanning microscope (ISM)’s spatial resolution. ISM, itself an emerging technique, uses a confocal microscope in which a detector array replaces a single detector. In SOFISM, correlations of intensities detected by multiple detectors are computed. In…READ MORE