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Traditional optical microscopes require constant adjustments to bring a sample into focus. To see a small feature with details, researchers must use a high-resolution objective lens with a reduced field of view. The trade-off between resolution and imaging area is a major inconvenience for life scientists and pathologists who rely on microscopy to analyze and diagnose disease, because prepared tissue samples have dimensions in the centimeter range1.
It turns out, however, that this inherent limitation can be overcome by using lensless equipment. Rather than using lenses to magnify the object image, a thin diffuser can be placed between the specimen and the image sensor in a lensless setting (Figure 1). The diffuser is then randomly…READ MORE