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    Deconvolution makes it clear

    Article obtained from BioPhotonics RSS Feed.

    Diffraction-limited microscopy presents a challenge in the laboratory because, depending on the depth desired, structures can be blurred or resolved in areas that change the location of the final image. This is where deconvolution can be a valuable tool, building on the point spread function (PSF) of the microscope and generating iterations of an image until it resembles the true state of a specimen.

    Commercial software allows a variety of algorithms to be applied with confocal, multiphoton, and superresolution microscopes. These algorithms, which vary depending on the PSF and noise function, can measure planes in a 3D image or begin with the Fourier transform of an image and divide it by the Fourier transform of the PSF.


    Nov, 19 2019 |

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