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    Correction Systems for Deep Imaging Drive More Precise Views

    Article obtained from Photonics RSS Feed.

    Multiphoton microscopy is widely regarded as the primary technique for deep imaging in live tissue. However, image brightness and resolution are typically poorer at deep sites because of increased optical spherical aberration compared to superficial locations. This makes it difficult to study fine structures, such as dendritic spines in deep brain regions.

    Microscope objectives equipped with adjustable correction collars can compensate for spherical aberration and improve resolution by moving internal lens elements. Motorized correction collars facilitate more precise and convenient operation compared to direct manual control. Often, however, corrections are only applied at a static depth within the specimen.

    An automated…

    May, 17 2019 |

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