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Conventional bright-field microscopy is the standard for biomedical imaging because it provides cost-effective clinical analysis. However, for researchers and clinicians to assess tissue images accurately, years or even decades of experience are needed. One way to increase selectivity and specificity in life science microscopy is to exploit exogeneous labels; for example, by using fluorophore dyes to enhance contrast in histopathology. Exogenous labels have various drawbacks, as they may perturb the system — especially when used for in vivo applications — and they are not universal, in the sense that laborious tests and developments have to be performed before validating a label methodology.
The Raman effect provides high…READ MORE