Illumination 101 Guide
by
IDEX Health & Science
Navigating the Throughput Challenge
Larger fields and faster scans demand more from illumination. As imaging instruments transition to larger fields of view and rapid line-scanning, the margin for error in lighting disappears. To maintain data integrity in high-speed fluorescence microscopy, you need more than just power—you need higher radiance, multi-wavelength precision, and speckle-free uniformity.
Building on the decades-long legacy of Melles Griot® Optical Systems, this guide provides the technical foundation for optimizing the optical path for demanding, high-throughput applications.
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Tell Us About Your ProjectInside the Guide:
We address the core challenges of uniform multi-wavelength illumination, high-power delivery, and system integration with a suite of advanced technologies:
- Medium to High-Power Reliable Laser Diodes or LED Light Engines
- Flat-Top, Speckle-Free Widefield Illumination
- Beam Shaping to Match Sample Field of View and Sensor
- Advanced Laser Modulation and Thermal Management
- Compact, Drop-In Footprint Free Space or Fiber Delivery
- Compatibility with IDEX Health & Science XPLAN™ Objectives and Laser Autofocus
Critical for Applications Including:
Next-Generation Sequencing | Spatial Biology | Digital Pathology | Next-Gen Proteomics | Multi-Omics | High-Throughput Screening | Super-Resolution Microscopy | Optical Metrology | Machine Vision | Animal Imaging | Multiplex qPCR