Technique Enables In Vivo Vascular Imaging at Single-Cell Resolution
Sep 22, 2022, 03:00 AM
A two-photon fluorescence imaging method developed by researchers at the University of Hong Kong and the University of California, Berkeley was able to image the flow of individual blood cells at 1000 2D frames and 1,000,000 line-scans per second in the brains of awake mice.
Such rapid measurements go beyond the reach of conventional two-photon microscopy methods to enable the study of blood flow at the single-cell level in large blood vessels. The ultrafast two-photon fluorescence imaging technique could help scientists better understand how energy is distributed and regulated in both healthy and diseased brains, by revealing blood flow changes at the level of individual blood vessels and across the larger vessel network within the...