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A Multiphoton Microscope Enables Portable 3D Biological Imaging

Sep 16, 2022, 11:21 AM by User Not Found

For decades, optical microscopy has provided the mechanism with which to image cells and tissue for the purposes of cancer research, digital pathology, and the study of the brain. Samples are typically studied in frozen sections. Most of these systems use confocal microscopy and single-photon excitation, typically from a continuous-wave light source, to probe the sample. Multiphoton microscopy is used when deep-tissue and cellular-level resolution are desired to preserve the image of the tissue in its native environment. In laser scanning multiphoton microscopes, light from an ultrafast laser is tightly focused and scanned across the sample using fast mirrors. An image is created by detecting the fluorescence signal intensity at each point...
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