Melles Griot® High Power Laser Engines
Powering High-Throughput Next-Generation Imaging
The Melles Griot® High Power Laser Engine from IDEX Health & Science is engineered to address the growing need for powerful, cost-effective illumination in fluorescence-based imaging instruments. Why High Power Illumination? Companies with High-throughput application platforms in areas like Next Generation Sequencing, Spatial Biology, Next Generation Proteomics, and High Content Screening are striving for even higher throughput.
To keep up with the demand for higher throughput, imaging systems are using larger fields of view for "step-and-shoot" imaging or ultra-fast line scanning with TDI sensors. Both of these methods require significantly higher power illumination to achieve the power densities needed for effective fluorescence detection. While competitors use DPSS technology for these high power systems, they are often too expensive. Our solution provides a cost-effective alternative to these systems.
The Melles Griot High Power Laser Engine is a compact, cost-effective, multi-wavelength light engine that delivers high irradiance and photon efficiency. It is specifically designed to enable higher throughput in genomics, transcriptomics, and proteomics workflows without the high cost of DPSS solutions.
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Market Need for High Power Illumination
Higher Throughput Imaging can be achieved in two ways.
The first method (Figure 1) applies to the step and shoot imaging using a 2D sensor where a rectangular or square area is illuminated and the fluorescence emission is imaged. The next field of view is then imaged, and this process is repeated until the whole sample is imaged. Then the individual tiles can be stitched together to get data from the whole sample. This process, depending on the application, the sample, the chemistry, and the number of targets to be imaged, can take several hours, or even days, to complete. To increase the throughput, one simply images a larger field of view with a large field of view objective and captures the image with a larger sensor. As the larger field of view is illuminated, the laser power must be increased in order to maintain sufficient irradiance.
The second method (Figure 2) uses High speed line scan imaging with a TDI sensor (time delay integration). In this method a Line field of view, typical 10’s of microns in width and a mm in length, is illuminated. The sample stage is moved across at high speed, and the line sensor captures the image in a continuous scan. In this case, since the camera is only seeing the sample for a very short time, more laser power is needed to compensate for shorter exposure times.
- Current high power illumination solutions in the market use high power DPPS Laser technology (Diode pumped solid state) which are expensive and have a large footprint.
- Current IDEX Health & Science laser diode solutions only produce power in the range of 500 mW up to 2 W maximum
There is, therefore, a market need for a cost-effective high power (> 2 W), multi wavelength, compact Laser engine solution.
Compact and Cost-Effective Laser Engine Solution
Unlock unmatched imaging performance with our compact, cost-effective, multi-wavelength flat-top light engines, designed for high irradiance and maximum photon efficiency in next-gen high-throughput, high-resolution, multi-omics 2D and TDI line imaging systems.
Laser Engine Options
| ~5W, ~10W, ~15W | Rectangular, or Line Flat-top Speckle-free Beam | Air- or Water-cooled Thermal Management |
| Single, Dual, or Triple Wavelength Light Engines | Compact Modules | Analog or Digital Control |
Precision and Power
- Spectral Purity – very narrow bandwidth +/- 2-5 nm Vs 20-30 nm Bandwidth with LED”s
- Intensity - High peak power, ideal for deep excitation – Lower LED intensity may struggle with weak fluorophores
- Beam Quality – Highly collimated, easily focused – LED Diffused, harder to shape precisely
- Modulation speed – Faster modulation of lasers especially for dynamic studies
Key Features and Benefits
- > 5 W Power: Illuminate a larger field of view or reduce exposure time and capture up to 4x more data per image for faster throughput and faster run times.
- Up to 95% even Illumination Across the Sample: 100% usable field of view compared to ~30% with others. Quantitative Accuracy with consistent excitation across all wavelengths.
- Red, Green, and Blue Wavelengths: Six excitation wavelengths to cover commonly used fluorophores.
- Flexible Beam Shaping: Easily configure your sample area illumination to match your choice of 2D or TDI sensor formats and maximize your photon budget. Compatible with XPLAN lenses.
- Thermal Management: Air or water-cooled options including Thermoelectric Cooling (TEC) to ensure stable temperature control, for wavelength stability, consistent power output performance and longer system life.
- Compact, Drop-in Footprint: Designed to save space and simplify OEM integration.
- Integrated Drive Electronics: Drivers built for complex laser cycles and thermal control, critical for the high power diode lasers.
- Development Budget Friendly: Access high power semi-custom laser light engines with laser modulation and heat management without upfront engineering (NRE) fees.
Range of Laser Wavelengths
Flexible Choice of Fluorophores
Melles Griot High Power Laser Engines cover a range of wavelengths, which can be used with a range of commonly used fluorophores. Additional wavelengths can be added for custom OEM projects.
Examples of fluorophores excited by the wavelengths offered
| Laser Wavelength | Fluorophore | Excitation (nm) | Emission (nm) |
|---|---|---|---|
| 455 nm – 465 nm | Pacific Blue | ~404 – 410 | ~455 |
| Hoechst 33258/33342 | ~352 | ~455 | |
| BOBO-1 / POPO-1 | ~433 – 435 | ~457 – 458 | |
| CFP / eCFP | ~434 – 437 | ~474 – 476 | |
| 488 nm | FITC / FAM | ~495 | ~517 – 520 |
| Alexa Fluor 488 | ~493 | ~520 | |
| GFP / eGFP | ~488 – 489 | ~509 | |
| SYTO 13 / SYBR Green | ~488 – 498 | ~506 – 522 | |
| Fluo-4 / Calcein | ~494 | ~514 – 516 | |
| 525 nm – 532 nm | AlexaFluor 532 | ~531 | ~554 |
| Cy3 | ~522 | ~565 | |
| TRITC / TAMRA | ~541 – 542 | ~572 | |
| Phycoerythrin (PE) | ~545 – 565 | ~576 – 578 | |
| 638 nm | Cy5 | ~650 | ~667 |
| Alexa Fluor 647 | ~650 | ~665 | |
| APC (Allophycocyanin) | ~650 | ~660 | |
| DRAQ5 | ~646 | ~681 |
Combine the Melles Griot High Power Laser Engine with XPLAN
Our High Power Laser Engines and XPLAN objectives and tube lenses work together to accommodate a range of sensor formats for your high throughput high resolution instrument design.
Product Specifications
Wavelength and Power Options
We offer a range of single, dual, and triple wavelength options with power levels up to 15 W.
Single Wavelength Options
| Power | 638 | 532 | 525 | 465 | 455 | 488 |
|---|---|---|---|---|---|---|
| ~5 W | ✔ | ✔ | ✔ | ✔ | ✔ | ✔ |
| ~10 W | ✔ | ✔ | ✔ | ✔ | ✔ | — |
| ~15 W | ✔ | ✔ | ✔ | ✔ | ✔ | — |
Dual Wavelength Options
| POWER | 638 | 532 | 638 | 525 | 532 | 465/455 | 525 | 465/455 | 532 | 488 | 525 | 488 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ~5 W | ✔ | ✔ | ✔ | ✔ | ✔ | ✔ | ||||||
| ~10 W | ✔ | ✔ | ✔ | ✔ | — | — | ||||||
| ~15 W | — | ✔ | ✔ | ✔ | — | — |
Triple Wavelength Options
| POWER | 638 | 532 | 465/455 | 638 | 525 | 465/455 | 638 | 532 | 488 | 638 | 525 | 488 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ~5 W | ✔ | ✔ | ✔ (488 2 W) | ✔ (488 2 W) | ||||||||
| ~10 W | ✔ | ✔ | — | — | ||||||||
| ~15 W | — | ✔ | — | — | ||||||||
Add-on Options
| LASER CONTROL OPTIONS |
| Analog Controller Unit |
| Built-In Digital Controller |
| THERMAL MANAGEMENT OPTIONS |
| TEC Control Assembly |
| Fan Cooling |
| Water Cooling |
| BEAM SHAPING OPTIONS |
| Square FOV Format |
| Rectangular FOV Format |
| Line Beam Shaping - Single Beam |
| Line Beam Shaping - Dual Beam |
The Next Generation of Imaging Illumination
Don't let cost or complexity hinder your research. At IDEX Health & Science, we are powering precision in High-Throughput Next-Generation Imaging. Our Melles Griot High Power Laser Engine is a testament to that mission, offering a high power, multi-wavelength light engine that delivers a flat-top beam with unmatched photon efficiency. Unlock unmatched imaging performance and power your next breakthrough with a cost-effective, quick-to-bread solution built for speed and precision. Discover how our technology can illuminate your discoveries.