This filter set is ideal for use with Yellow Fluorescent Protein (YFP) and other similar fluorophores. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with similar contrast.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,324.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-535/22-25
FL-003976 / 0
- Tavg > 90% 524.7 – 546.3 nm
- Center Wavelength of 535.5 nm and GMBW of 21.6 nm
- FWHM of 27.1 nm
FF01-497/16-25
FL-004378 / 0
- Tavg > 90% 489.6 – 505.2 nm
- Center Wavelength of 497.4 nm and GMBW of 15.6 nm
- FWHM of 20.8 nm
FF516-Di01-25x36
FL-006799 / 0
- Dichroic Beamsplitter with edge at 516 nm
- Ravg > 90% 490 – 510 nm
- Tavg > 90% 520 – 700 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 6-JOE | 520 | 548 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 514 | 518 | 543 |
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 520 | 516 | 538 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY R6G | 528 | 547 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| CFP2 | 490 | 510 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| DiA | 457 | 586 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| Emerald | 491 | 511 |
|
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| Eosin | 525 | 546 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| FM 1-43 | 473 | 579 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
|
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| TO-PRO-1 | 515 | 531 |
|
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| TOTO-1 | 514 | 531 |
|
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| TurboYFP | 525 | 538 |
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| Venus | 516 | 528 |
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
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| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-535/22-25
FL-003976 / 0
- Tavg > 90% 524.7 – 546.3 nm
- Center Wavelength of 535.5 nm and GMBW of 21.6 nm
- FWHM of 27.1 nm
FF01-497/16-25
FL-004378 / 0
- Tavg > 90% 489.6 – 505.2 nm
- Center Wavelength of 497.4 nm and GMBW of 15.6 nm
- FWHM of 20.8 nm
FF516-Di01-25x36
FL-006799 / 0
- Dichroic Beamsplitter with edge at 516 nm
- Ravg > 90% 490 – 510 nm
- Tavg > 90% 520 – 700 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |