BrightLine® single-band filter set, optimized for Yellow Fluorescent Protein and other like fluorophores

Semrock Part Number: YFP-2427B-NTE

IDEX Part Number: S-001997  /

Semrock

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring YFP and its variants – no more compromising or exchanging filter sets for different YFP experiments.

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,489.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-500/24-25

FL-004379 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 488 – 512 nm
  • Center Wavelength of 500 nm and GMBW of 24 nm
  • FWHM of 29.7 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

FF520-Di02-25x36

FL-007023 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 520 nm
  • Ravg > 98% 350 – 512 nm
  • Tavg > 93% 528.5 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Acridine Orange (+DNA) 502 526
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY R6G 528 547
Calcein 494 514
Calcium Green-1 507 529
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DiA 457 586
DY-505-Phalloidin 503 530
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
mOrange 546 562
mOrange2 551 565
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-triglyceride 510 583
OFP 547 567
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
POPO-3 533 574
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
YFP (yellow GFP) 513 530
YO-PRO-1 491 506
YOYO-1 491 508
ZsYellow1 529 539

Related Products

FF01-500/24-25

FL-004379 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 488 – 512 nm
  • Center Wavelength of 500 nm and GMBW of 24 nm
  • FWHM of 29.7 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

FF520-Di02-25x36

FL-007023 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 520 nm
  • Ravg > 98% 350 – 512 nm
  • Tavg > 93% 528.5 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.