BrightLine Basic single-band filter set, optimized for Wild-type GFP and other like fluorophores

Semrock Part Number: WGFP-A-Basic-OFF

IDEX Part Number: S-001991  /

Semrock

This filter set is ideal for use with wild-type Green Fluorescent Protein (wtGFP), which is blue-shifted relative to the most common forms of GFP. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with better contrast.

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  • 1,374.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-445/45-25

FL-004670 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 423 – 468 nm
  • Center Wavelength of 445.5 nm and GMBW of 45 nm
  • FWHM of 49.5 nm

FF01-510/42-25

FL-004677 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 489.5 – 531.2 nm
  • Center Wavelength of 510.3 nm and GMBW of 41.7 nm
  • FWHM of 47 nm

FF482-Di01-25x36

FL-006842 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 482 nm
  • Ravg > 90% 415 – 470 nm
  • Tavg > 90% 490 – 720 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BD Horizon V500 410 500
BOBO™-1 461 484
Cascade Yellow™ 399 549
CellTracker Violet BMQC+GSH 406 526
Cerulean 434 473
CFP (ECFP) 433 475
CFP2 490 510
Cy2™ 492 507
DEAC 432 472
DY-415 418 470
ECFP 435 475
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
evoglow-Bs1 449 496
GFP (EGFP) 489 511
Lucifer yellow 428 544
LysoSensor Green 448 502
mBBr+GSH 394 490
mHoneyDew 478 562
MitoTracker™ Green 490 512
mTFP1 462 492
NBD-X (MeOH) 467 538
PKH67 488 500
POPO-1 433 457
Qdot® 525 Nanocrystals 300 525
sgGFP™ (super glow GFP) 472 506
SpectrumAqua 434 481
SYTO 45 451 485
SYTO 9 483 500
SYTOX Blue 444 470
TagCFP 458 480
ThiolTracker Violet+GSH 404 526
TurboGFP 482 503
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

Related Products

FF01-445/45-25

FL-004670 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 423 – 468 nm
  • Center Wavelength of 445.5 nm and GMBW of 45 nm
  • FWHM of 49.5 nm

FF01-510/42-25

FL-004677 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 489.5 – 531.2 nm
  • Center Wavelength of 510.3 nm and GMBW of 41.7 nm
  • FWHM of 47 nm

FF482-Di01-25x36

FL-006842 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 482 nm
  • Ravg > 90% 415 – 470 nm
  • Tavg > 90% 490 – 720 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.