This filter set is ideal for use with wild-type Green Fluorescent Protein (wtGFP), which is blue-shifted relative to the most common forms of GFP. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with better contrast.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-445/45-25
FL-004670 / 0
- Tavg > 90% 423 – 468 nm
- Center Wavelength of 445.5 nm and GMBW of 45 nm
- FWHM of 49.5 nm
FF01-510/42-25
FL-004677 / 0
- Tavg > 90% 489.5 – 531.2 nm
- Center Wavelength of 510.3 nm and GMBW of 41.7 nm
- FWHM of 47 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| DEAC | 432 | 472 |
|
|
| DY-415 | 418 | 470 |
|
|
| ECFP | 435 | 475 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| mTFP1 | 462 | 492 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| PKH67 | 488 | 500 |
|
|
| POPO-1 | 433 | 457 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| TagCFP | 458 | 480 |
|
|
| ThiolTracker Violet+GSH | 404 | 526 |
|
|
| TurboGFP | 482 | 503 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-445/45-25
FL-004670 / 0
- Tavg > 90% 423 – 468 nm
- Center Wavelength of 445.5 nm and GMBW of 45 nm
- FWHM of 49.5 nm
FF01-510/42-25
FL-004677 / 0
- Tavg > 90% 489.5 – 531.2 nm
- Center Wavelength of 510.3 nm and GMBW of 41.7 nm
- FWHM of 47 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LL01-248-12.5
FL-007859