This filter set is ideal for use with wild-type Green Fluorescent Protein (wtGFP), which is blue-shifted relative to the most common forms of GFP. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with better contrast.
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FF01-445/45-25
FL-004670 / 0
- Tavg > 90% 423 – 468 nm
- Center Wavelength of 445.5 nm and GMBW of 45 nm
- FWHM of 49.5 nm
FF01-510/42-25
FL-004677 / 0
- Tavg > 90% 489.5 – 531.2 nm
- Center Wavelength of 510.3 nm and GMBW of 41.7 nm
- FWHM of 47 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| DEAC | 432 | 472 |
|
|
| DY-415 | 418 | 470 |
|
|
| ECFP | 435 | 475 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| mTFP1 | 462 | 492 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| PKH67 | 488 | 500 |
|
|
| POPO-1 | 433 | 457 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| TagCFP | 458 | 480 |
|
|
| ThiolTracker Violet+GSH | 404 | 526 |
|
|
| TurboGFP | 482 | 503 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-445/45-25
FL-004670 / 0
- Tavg > 90% 423 – 468 nm
- Center Wavelength of 445.5 nm and GMBW of 45 nm
- FWHM of 49.5 nm
FF01-510/42-25
FL-004677 / 0
- Tavg > 90% 489.5 – 531.2 nm
- Center Wavelength of 510.3 nm and GMBW of 41.7 nm
- FWHM of 47 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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