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BrightLine® 2.0 single-band filter set, optimized for TxRed and other like fluorophores

Semrock Part Number: TxRed-4040D-000

IDEX Part Number: S-443519  /

Semrock

BrightLine™ 2.0 filter sets introduces a unified, improved design that delivers enhanced contrast and leverages the latest advances in coating technologies across this industry-leading filter family. The fluorescence bandpass filters and sets are meticulously optimized for seamless integration across a wide range of fluorescence instruments. With its all-thin-film, hard-coated construction, BrightLine 2.0 sets a new standard in performance and reliability.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 908.43 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

FF02-562/40-25

FL-442966

Each
  • Tavg > 95% 542 – 582 nm
  • Center Wavelength of 562 nm and GMBW of 40 nm
  • FWHM of 45.5 nm

FL-442966

FF01-622/44-25

FL-442967

Each
  • Tavg > 95% 600 – 644 nm
  • Center Wavelength of 622 nm and GMBW of 44 nm
  • FWHM of 51.6 nm

FL-442967

FF591-Di01-25x36

FL-442962

Each
  • Dichroic Beamsplitter with edge at 591 nm
  • Ravg > 98% 350 – 582 nm
  • Tavg > 95% 600 – 1100 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-442962

Optical Specifications Value
Blocking BrightLine® 2.0 filters have blocking far exceeding OD 8 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine® 2.0 filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
BOBO™-3 570 605
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cy3.5™ 578 591
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LysoTracker Red 573 592
mApple 566 594
mCherry 587 610
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
OFP 547 567
pHrodo™, succinimidyl ester 560 587
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhodamine Red-X 573 591
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TurboFP635(Katushka) 591 638
TurboRFP 553 573
X-Rhod-1 Indicator 580 601
YOYO-3 611 631

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

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