This filter set is ideal for use with Texas Red® and other red fluorophores, including the monomeric fluorescent protein mCherry. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
---|---|---|---|---|
2-dodecylresorufin-lipid | 582 | 595 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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AsRed 2 | 576 | 592 |
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ATTO 565 | 563 | 592 |
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ATTO 590 | 594 | 624 |
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ATTO 594 | 601 | 624 |
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BOBO™-3 | 570 | 605 |
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BODIPY TR-X phallacidin | 590 | 621 |
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Calcium Crimson | 589 | 609 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTracker Red CMTPX | 586 | 614 |
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Cy3.5™ | 578 | 591 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-590 | 582 | 599 |
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DyLight 594 | 593 | 618 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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HcRed1 | 588 | 618 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluor™ 594 | 592 | 616 |
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KFP-Red | 575 | 599 |
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LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
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LysoTracker Red | 573 | 592 |
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mApple | 566 | 594 |
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mCherry | 587 | 610 |
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MitoTracker™ Red | 578 | 598 |
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mKate2 | 588 | 633 |
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mPlum | 589 | 649 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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Nile red-phospolipid | 553 | 637 |
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OFP | 547 | 567 |
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pHrodo™, succinimidyl ester | 560 | 587 |
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Propidium Iodide (PI) | 305 | 617 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Qdot® 625 Nanocrystals | 300 | 621 |
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ReAsH-CCPGCC | 592 | 606 |
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Red 580 | 580 | 603 |
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Resorufin | 571 | 584 |
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Rhodamine Red-X | 573 | 591 |
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SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 488nm (ph 9.0) | 576 | 638 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SNARF-1 514nm (ph 9.0) | 576 | 638 |
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SpectrumOrange | 554 | 587 |
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SpectrumRed | 587 | 615 |
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Sulforhodamine 101-EtOH | 578 | 593 |
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Tetramethylrhodamine dextran | 554 | 582 |
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Texas Red dextran | 592 | 613 |
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Texas Red DHPE | 584 | 608 |
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Texas Red® | 592 | 614 |
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TurboFP635(Katushka) | 591 | 638 |
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TurboRFP | 553 | 573 |
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X-Rhod-1 Indicator | 580 | 601 |
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YOYO-3 | 611 | 631 |
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |