BrightLine® single-band filter set, optimized for Tryptophan and other like fluorophores

Semrock Part Number: TRP-A-OFF

IDEX Part Number: S-000586  /

Semrock

Unique UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blocking that rival high-performance visible filters. The filters in this set are ideal for imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. Note: This set is optimized for systems using a light source (such as a UV LED or filtered Xe arc lamp) with no near-infrared (near-IR) intensity and/or a detector (such as a photomultiplier tube or IR-filtered CCD camera) that is not sensitive to near-IR light. It is not designed to block near-IR light – for applications involving a light source with appreciable near-IR intensity and a near-IR-sensitive detector, contact Semrock.

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  • 1,924.00 USD Each
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FF01-280/20-25

FL-004465 / 0

Out of Stock Backordered In Stock
  • Tavg > 65% 270 – 290 nm
  • Center Wavelength of 280 nm and GMBW of 20 nm
  • FWHM of 22 nm

FF01-357/44-25

FL-004579 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 335 – 379 nm
  • Center Wavelength of 357 nm and GMBW of 44 nm
  • FWHM of 47.7 nm

FF310-Di01-25x36

FL-007083 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 310 nm
  • Ravg > 98% 255 – 295 nm
  • Tavg > 90% 315 – 600 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Naphthalene 275 322
Purine 272 405
Tryptophan 295 340

Related Products

FF01-280/20-25

FL-004465 / 0

Out of Stock Backordered In Stock
  • Tavg > 65% 270 – 290 nm
  • Center Wavelength of 280 nm and GMBW of 20 nm
  • FWHM of 22 nm

FF01-357/44-25

FL-004579 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 335 – 379 nm
  • Center Wavelength of 357 nm and GMBW of 44 nm
  • FWHM of 47.7 nm

FF310-Di01-25x36

FL-007083 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 310 nm
  • Ravg > 98% 255 – 295 nm
  • Tavg > 90% 315 – 600 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.